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celltiter-96® aqueous one-solution cell proliferation assay mts reagent  (Promega)

 
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    Promega celltiter-96® aqueous one-solution cell proliferation assay mts reagent
    Celltiter 96® Aqueous One Solution Cell Proliferation Assay Mts Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/celltiter-96%C2%AE+aqueous+one-solution+cell+proliferation+assay+mts+reagent/celltiter+glo+luminescent+cell+viability+assay/pmc12274511-223-74-83
    Average 90 stars, based on 1 article reviews
    celltiter-96® aqueous one-solution cell proliferation assay mts reagent - by Bioz Stars, 2026-11
    90/100 stars

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    Incubation:

    Article Title: Inhibition of fatty acid binding protein suppresses pancreatic cancer progression and metastasis
    Article Snippet: Cell suspensions (3000 cells/100 μl) were added to each well in a 96-multiwell culture plate and incubated at 37 °C for 24 h. HTS (0.03, 0.06, 0.12, 0.24, 0.49, 0.98, 2.0, 3.9, 7.8, 15.6, 31.3, 62.5, 125, 250 μM), FABP4 (100 ng/ml) and FABP4 + HTS (30 μM) were subsequently added to each well, and the cells were incubated for a further 48 h. At the end of the culture period, 10 μl of CellTiter-96 ® Aqueous One-Solution Cell Proliferation Assay MTS reagent (Promega, Madison, WI, USA) was added to each 100 μl of culture media, which were incubated for 2 h. Absorbance at 490 nm was measured with a multimode reader and the results were expressed as the percentage viable with respect to the untreated control.

    Proliferation Assay:

    Article Title: Inhibition of fatty acid binding protein suppresses pancreatic cancer progression and metastasis
    Article Snippet: Cell suspensions (3000 cells/100 μl) were added to each well in a 96-multiwell culture plate and incubated at 37 °C for 24 h. HTS (0.03, 0.06, 0.12, 0.24, 0.49, 0.98, 2.0, 3.9, 7.8, 15.6, 31.3, 62.5, 125, 250 μM), FABP4 (100 ng/ml) and FABP4 + HTS (30 μM) were subsequently added to each well, and the cells were incubated for a further 48 h. At the end of the culture period, 10 μl of CellTiter-96 ® Aqueous One-Solution Cell Proliferation Assay MTS reagent (Promega, Madison, WI, USA) was added to each 100 μl of culture media, which were incubated for 2 h. Absorbance at 490 nm was measured with a multimode reader and the results were expressed as the percentage viable with respect to the untreated control.

    Control:

    Article Title: Inhibition of fatty acid binding protein suppresses pancreatic cancer progression and metastasis
    Article Snippet: Cell suspensions (3000 cells/100 μl) were added to each well in a 96-multiwell culture plate and incubated at 37 °C for 24 h. HTS (0.03, 0.06, 0.12, 0.24, 0.49, 0.98, 2.0, 3.9, 7.8, 15.6, 31.3, 62.5, 125, 250 μM), FABP4 (100 ng/ml) and FABP4 + HTS (30 μM) were subsequently added to each well, and the cells were incubated for a further 48 h. At the end of the culture period, 10 μl of CellTiter-96 ® Aqueous One-Solution Cell Proliferation Assay MTS reagent (Promega, Madison, WI, USA) was added to each 100 μl of culture media, which were incubated for 2 h. Absorbance at 490 nm was measured with a multimode reader and the results were expressed as the percentage viable with respect to the untreated control.



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